Discovery of estrogen receptor alpha target genes and response elements in breast tumor cells.

ABSTRACT:

Background

Estrogens and their receptors are important in human development, physiology and disease. In this study, we utilized an integrated genome-wide molecular and computational approach to characterize the interaction between the activated estrogen receptor (ER) and the regulatory elements of candidate target genes.

Results

Of around 19,000 genes surveyed in this study, we observed 137 ER-regulated genes in T-47D cells, of which only 89 were direct target genes. Meta-analysis of heterogeneous in vitro and in vivo datasets showed that the expression profiles in T-47D and MCF-7 cells are remarkably similar and overlap with genes differentially expressed between ER-positive and ER-negative tumors. Computational analysis revealed a significant enrichment of putative estroge

SUBMITTER: Lin CY 

PROVIDER: S-EPMC522873 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature