A catalog of human cDNA expression clones and its application to structural genomics.

ABSTRACT: We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.

SUBMITTER: Bussow K 

PROVIDER: S-EPMC522878 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature