Lactobacillus plantarum bacteriophage LP65: a new member of the SPO1-like genus of the family Myoviridae.

ABSTRACT: The virulent Lactobacillus plantarum myophage LP65 was isolated from industrial meat fermentation. Tail contraction led to reorganization of the tail sheath and the baseplate; a tail tube was extruded. In ultrathin section the phage adsorbed via its baseplate to the exterior of the cell, while the tail tube tunneled through the thick bacterial cell wall. Convoluted membrane structures were induced in the infected cell. Progeny phage was detected 100 min postinfection, and lysis occurred after extensive digestion of the cell wall. Sequence analysis revealed a genome of 131,573 bp of nonredundant DNA. Four major genome regions and a large tRNA gene cluster were observed. One module corresponded to DNA replication genes. Helicase/primase and two replication/recombination enzymes represented t

SUBMITTER: Chibani-Chennoufi S 

PROVIDER: S-EPMC523202 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature