Characterization of nutrient-induced dispersion in Pseudomonas aeruginosa PAO1 biofilm.

ABSTRACT: The processes associated with early events in biofilm formation have become a major research focus over the past several years. Events associated with dispersion of cells from late stage biofilms have, however, received little attention. We demonstrate here that dispersal of Pseudomonas aeruginosa PAO1 from biofilms is inducible by a sudden increase in carbon substrate availability. Most efficient at inducing dispersal were sudden increases in availability of succinate > glutamate > glucose that led to approximately 80% reductions in surface-associated biofilm biomass. Nutrient-induced biofilm dispersion was associated with increased expression of flagella (fliC) and correspondingly decreased expression of pilus (pilA) genes in dispersed cells. Changes in gene expression associated with di

SUBMITTER: Sauer K 

PROVIDER: S-EPMC523207 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature