Impact on genetic networks in human macrophages by a CCR5 strain of human immunodeficiency virus type 1.

ABSTRACT: Human immunodeficiency virus type 1 (HIV-1) impacts multiple lineages of hematopoietic cells, including lymphocytes and macrophages, either by direct infection or indirectly by perturbations of cell networks, leading to generalized immune deficiency. We designed a study to discover, in primary human macrophages, sentinel genetic targets that are impacted during replication over the course of 7 days by a CCR5-using virus. Expression of mRNA and proteins in virus- or mock-treated macrophages from multiple donors was evaluated. Hierarchical agglomerative cluster analysis grouped into distinct temporal expression patterns >900 known human genes that were induced or repressed at least fourfold by virus. Expression of more than one-third of the genes was induced rapidly by day 2 of infection, wh

SUBMITTER: Coberley CR 

PROVIDER: S-EPMC523249 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature