Propagation of measurement accuracy to biomass soft-sensor estimation and control quality.

ABSTRACT: In biopharmaceutical process development and manufacturing, the online measurement of biomass and derived specific turnover rates is a central task to physiologically monitor and control the process. However, hard-type sensors such as dielectric spectroscopy, broth fluorescence, or permittivity measurement harbor various disadvantages. Therefore, soft-sensors, which use measurements of the off-gas stream and substrate feed to reconcile turnover rates and provide an online estimate of the biomass formation, are smart alternatives. For the reconciliation procedure, mass and energy balances are used together with accuracy estimations of measured conversion rates, which were so far arbitrarily chosen and static over the entire process. In this contribution, we present a novel strategy within t

SUBMITTER: Steinwandter V 

PROVIDER: S-EPMC5233751 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature