TFEB-mediated increase in peripheral lysosomes regulates store-operated calcium entry.

ABSTRACT: Lysosomes are membrane-bound organelles mainly involved in catabolic processes. In addition, lysosomes can expel their contents outside of the cell via lysosomal exocytosis. Some of the key steps involved in these important cellular processes, such as vesicular fusion and trafficking, require calcium (Ca²⁺) signaling. Recent data show that lysosomal functions are transcriptionally regulated by transcription factor EB (TFEB) through the induction of genes involved in lysosomal biogenesis and exocytosis. Given these observations, we investigated the roles of TFEB and lysosomes in intracellular Ca²⁺ homeostasis. We studied the effect of transient modulation of TFEB expression in HeLa cells by measuring the cytosolic Ca²⁺ response after capacitative Ca^2+</

SUBMITTER: Sbano L 

PROVIDER: S-EPMC5233950 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature