Transcriptional pausing at the translation start site operates as a critical checkpoint for riboswitch regulation.

ABSTRACT: On the basis of nascent transcript sequencing, it has been postulated but never demonstrated that transcriptional pausing at translation start sites is important for gene regulation. Here we show that the Escherichia coli thiamin pyrophosphate (TPP) thiC riboswitch contains a regulatory pause site in the translation initiation region that acts as a checkpoint for thiC expression. By biochemically probing nascent transcription complexes halted at defined positions, we find a narrow transcriptional window for metabolite binding, in which the downstream boundary is delimited by the checkpoint. We show that transcription complexes at the regulatory pause site favour the formation of a riboswitch intramolecular lock that strongly prevents TPP binding. In contrast, cotranscriptional metabolite b

SUBMITTER: Chauvier A 

PROVIDER: S-EPMC5234074 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature