Differential expression of E-type prostanoid receptors 2 and 4 in microglia stimulated with lipopolysaccharide.

ABSTRACT:

Background

Cyclooxygenase-2 (COX-2) is induced under inflammatory conditions, and prostaglandin E₂ (PGE₂) is one of the products of COX activity. PGE₂ has pleiotropic actions depending on the activation of specific E-type prostanoid EP1-4 receptors. We investigated the involvement of PGE₂ and EP receptors in glial activation in response to an inflammatory challenge induced by LPS.

Methods

Cultures of mouse microglia or astroglia cells were treated with LPS in the presence or absence of COX-2 inhibitors, and the production of PGE₂ was measured by ELISA. Cells were treated with PGE₂, and the effect on LPS-induced expression of TNF-α messenger RNA (mRNA) and protein was studied in the presence or absence of drug

SUBMITTER: Bonfill-Teixidor E 

PROVIDER: S-EPMC5234110 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature