Evaluation of the Antibody in Lymphocyte Supernatant Assay to Detect Active Tuberculosis.

ABSTRACT:

Background

We aimed to evaluate the antibody in lymphocyte supernatant (ALS) assay as a biomarker to diagnose tuberculosis among adults from Tanzania with and without HIV.

Methods

Adults admitted with suspicion for tuberculosis had sputa obtained for GeneXpert MTB/RIF, acid-fast bacilli smear and mycobacterial culture; blood was obtained prior to treatment initiation and after 4 weeks. Adults hospitalized with non-infectious conditions served as controls. Peripheral blood mononuclear cells were cultured unstimulated for 72 hours. Anti-mycobacterial antibodies were measured from culture supernatants by ELISA, using BCG vaccine as the coating antigen. Median ALS responses were compared between cases and controls at baseline and between cases over time.

Results

Of 97 TB

SUBMITTER: Sariko M 

PROVIDER: S-EPMC5234774 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature