5.8S rRNA Sequence and Secondary Structure in Parabronema skrjabini and Related Habronematidae Species.

ABSTRACT:

Background

Genomic DNA was isolated from Parabronema skrjabini. rRNA region was amplified and sequenced.

Methods

The RNA secondary structure was predicted using mfold software ( http://mfold.rit.albany.edu ). The secondary structure with bulge, hairpins, helices, interior, external and multi loops was predicted for 5.8srDNA of our sequence of P. skrjabini and a sequence of P. skrjabini and two species of Habronema (H. microstoma and H. muscae) in GenBank. RNA motifs were predicted by MEME program version 4.10.2.

Results

The length of 5.8S rRNA sequence for P. skrjabini#1, P. skrjabini#2, H. microstoma and H. muscae was 158, 156, 127 and 127bp, and the DG required for the formation of the secondary s

SUBMITTER: Hasheminasab SS 

PROVIDER: S-EPMC5236104 | biostudies-literature | 2016 Apr-Jun

REPOSITORIES: biostudies-literature