The acute inhibition of enteric glial metabolism with fluoroacetate alters calcium signaling, hemichannel function, and the expression of key proteins.

ABSTRACT: Glia play key roles in the regulation of neurotransmission in the nervous system. Fluoroacetate (FA) is a metabolic poison widely used to study glial functions by disrupting the tricarboxylic acid cycle enzyme aconitase. Despite the widespread use of FA, the effects of FA on essential glial functions such as calcium (Ca²⁺) signaling and hemichannel function remain unknown. Therefore, our goal was to assess specifically the impact of FA on essential glial cell functions that are involved with neurotransmission in the enteric nervous system. To this end, we generated a new optogenetic mouse model to study specifically the effects of FA on enteric glial Ca²⁺ signaling by crossing PC::G5-tdTomato mice with Sox10::creER^T2 mice. FA did not change the peak glial C

SUBMITTER: McClain JL 

PROVIDER: S-EPMC5236112 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature