Recombinant adeno-associated virus carrying thymosin β₄ suppresses experimental colitis in mice.

ABSTRACT:

Aim

To investigate the protective effect of a recombinant adeno-associated virus carrying thymosin β₄ (AAV-Tβ₄) on murine colitis via intracolonic administration.

Methods

AAV-Tβ₄ was prepared and intracolonically used to mediate the secretory expression of Tβ₄ in mouse colons. Dextran sulfate sodium (DSS) was applied to induce the murine ulcerative colitis, and 2,4,6-trinitrobenzene sulfonic acid (TNBS) was used to establish a mouse colitis model resembling Crohn's disease. The disease severity and colon injuries were observed and graded to reveal the effects of AAV-Tβ₄ on colitis. The activities of myeloperoxidase (MPO) and superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were determined using

SUBMITTER: Zheng XY 

PROVIDER: S-EPMC5236504 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature