Pleurotus giganteus (Berk. Karun & Hyde), the giant oyster mushroom inhibits NO production in LPS/H₂O₂ stimulated RAW 264.7 cells via STAT 3 and COX-2 pathways.

ABSTRACT:

Background

Pleurotus giganteus (Berk. Karunarathna and K.D. Hyde), has been used as a culinary mushroom and is known to have medicinal properties but its potential as an anti-inflammatory agent to mitigate inflammation triggered diseases is untapped. In this study, the molecular mechanism underlying the protective effect of ethanol extract of P. giganteus (EPG) against lipopolysaccharide (LPS) and combination of LPS and hydrogen peroxide (H₂O₂)-induced inflammation on RAW 264.7 macrophages was investigated.

Method

The effect of EPG on nitric oxide (NO) production as an indicator of inflammation in RAW 264.7 macrophages was estimated based on Griess reaction that measures nitrite level. The expressions of inducible nitric oxide synthase (iNOS), cyclooxyg

SUBMITTER: Baskaran A 

PROVIDER: S-EPMC5237140 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature