Characterization of glomerular extracellular matrix by proteomic analysis of laser-captured microdissected glomeruli.

ABSTRACT: Abnormal extracellular matrix (ECM) remodeling is a prominent feature of many glomerular diseases and is a final common pathway of glomerular injury. However, changes in ECM composition accompanying disease-related remodeling are unknown. The physical properties of ECM create challenges for characterization of composition using standard protein extraction techniques, as the insoluble components of ECM are frequently discarded and many ECM proteins are in low abundance compared to other cell proteins. Prior proteomic studies defining normal ECM composition used a large number of glomeruli isolated from human kidneys retrieved for transplantation or by nephrectomy for cancer. Here we examined the ability to identify ECM proteins by mass spectrometry using glomerular sections compatible with

SUBMITTER: Hobeika L 

PROVIDER: S-EPMC5237413 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature