Biosynthesis of uniformly labeled ¹³C- and ¹⁴C-arachidonic acid in Mortierella alpina.

ABSTRACT: Arachidonic acid (ARA) is one of the most abundant polyunsaturated fatty acids (PUFAs) in the mammalian brain. Many enzymatically- and nonenzymatically-produced metabolic products have important and potent pharmacological properties. However, uniformly isotope labeled forms of ARA are not commercially available for studying the metabolic fates of ARA. This study describes a simple and efficient protocol for the biosynthesis of U-¹³C-ARA from U-¹³C-glucose, and U-¹⁴C-ARA from U-¹⁴C-glucose by Mortierella alpina. The protocols yield approximately 100nmol quantities of U-¹³C-ARA with an isotopic purity of 95% from a 500μl batch volume, and approximately 2μCi quantities of U-¹⁴C-ARA with an apparent specific activity in excess o

SUBMITTER: Lee JV 

PROVIDER: S-EPMC5237611 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature