Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis.

ABSTRACT:

Background

Basophil activation tests (BATs) have promise for research and for clinical monitoring of patients with allergies. However, BAT protocols vary in blood anticoagulant used and temperature and time of storage before testing, complicating comparisons of results from various studies.

Objective

We attempted to establish a BAT protocol that would permit analysis of blood within 24 hours of obtaining the sample.

Methods

Blood from 46 healthy donors and 120 patients with peanut allergy was collected into EDTA or heparin tubes, and samples were stored at 4°C or room temperature for 4 or 24 hours before performing BATs.

Results

Stimulation with anti-IgE or IL-3 resulted in strong upregulation of basophil CD203c in samples collected in EDTA or heparin, stored a

SUBMITTER: Mukai K 

PROVIDER: S-EPMC5237629 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature