Formate simultaneously reduces oxidase activity and enhances respiration in Campylobacter jejuni.

ABSTRACT: The foodborne microaerophilic pathogen, Campylobacter jejuni, possesses a periplasmic formate dehydrogenase and two terminal oxidases, which serve to metabolize formate and facilitate the use of oxygen as a terminal electron acceptor, respectively. Formate, a primary energy source for C. jejuni, inhibits oxidase activity in other bacteria. Here, we hypothesized that formate might affect both energy metabolism and microaerobic survival in C. jejuni. Subsequently, we showed that C. jejuni 81-176 (wildtype) exhibited enhanced chemoattraction to and respiration of formate in comparison to other organic acids. Formate also significantly increased C. jejuni's growth, motility, and biofilm formation under microaerobic (5% O₂) conditions. However, formate reduced oxidase activity under

SUBMITTER: Kassem II 

PROVIDER: S-EPMC5238407 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature