Quantifying the relative contributions of different solute carriers to aggregate substrate transport.

ABSTRACT: Determining the contributions of different transporter species to overall cellular transport is fundamental for understanding the physiological regulation of solutes. We calculated the relative activities of Solute Carrier (SLC) transporters using the Michaelis-Menten equation and global fitting to estimate the normalized maximum transport rate for each transporter (V_max). Data input were the normalized measured uptake of the essential neutral amino acid (AA) L-leucine (Leu) from concentration-dependence assays performed using Xenopus laevis oocytes. Our methodology was verified by calculating Leu and L-phenylalanine (Phe) data in the presence of competitive substrates and/or inhibitors. Among 9 potentially expressed endogenous X. laevis oocyte Leu transporter species, activitie

SUBMITTER: Taslimifar M 

PROVIDER: S-EPMC5238446 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature