Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion.

ABSTRACT: Recombinant protein production coopts the host cell machinery to provide high protein yields of industrial enzymes or biotherapeutics. However, since protein translation is energetically expensive and tightly controlled, it is unclear if highly expressed recombinant genes are translated as efficiently as host genes. Furthermore, it is unclear how the high expression impacts global translation. Here, we present the first genome-wide view of protein translation in an IgG-producing CHO cell line, measured with ribosome profiling. Through this we found that our recombinant mRNAs were translated as efficiently as the host cell transcriptome, and sequestered up to 15% of the total ribosome occupancy. During cell culture, changes in recombinant mRNA translation were consistent with changes in tra

SUBMITTER: Kallehauge TB 

PROVIDER: S-EPMC5238448 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature