Topoisomerase I inhibition leads to length-dependent gene expression changes in human primary astrocytes.

ABSTRACT: Topoisomerase I is required for the proper expression of long genes (> 100 kb) in mouse and human cortical neurons, including many candidate genes for autism spectrum disorder (ASD) [1]. Given the important role of astrocytes in brain development [2], we investigated whether long genes, including autism susceptibility genes, also require topoisomerase I expression in human primary astrocytes. We carried genome-wide expression profiling of cultured human primary astrocytes following treatment with the topoisomerase I inhibitor Topotecan, using Illumina microarrays. We identified several thousands of differentially expressed genes and confirmed that topoisomerase I inhibition affects gene expression in human primary astrocytes in a length-dependent manner. We also identified over 20 ASD-asso

SUBMITTER: Gokoolparsadh A 

PROVIDER: S-EPMC5238604 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature