5'-Terminal nucleotide variations in human cytoplasmic tRNAHisGUG and its 5'-halves.

ABSTRACT: Transfer RNAs (tRNAs) are fundamental adapter components of translational machinery. tRNAs can further serve as a source of tRNA-derived noncoding RNAs that play important roles in various biological processes beyond translation. Among all species of tRNAs, tRNA^HisGUG has been known to uniquely contain an additional guanosine residue at the -1 position (G_-1) of its 5'-end. To analyze this -1 nucleotide in detail, we developed a TaqMan qRT-PCR method that can distinctively quantify human mature cytoplasmic tRNA^HisGUG containing G_-1, U_-1, A_-1, or C_-1 or lacking the -1 nucleotide (starting from G₁). Application of this method to the mature tRNA fraction of BT-474 breast cancer cells revealed the presence o

SUBMITTER: Shigematsu M 

PROVIDER: S-EPMC5238791 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature