Fumarase activity: an in vivo and in vitro biomarker for acute kidney injury.

ABSTRACT: Renal ischemia/reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI), and at present, there is a lack of reliable biomarkers that can diagnose AKI and measure early progression because the commonly used methods cannot evaluate single-kidney IRI. Hyperpolarized [1,4-¹³C₂]fumarate conversion to [1,4-¹³C₂]malate by fumarase has been proposed as a measure of necrosis in rat tumor models and in chemically induced AKI rats. Here we show that the degradation of cell membranes in connection with necrosis leads to elevated fumarase activity in plasma and urine and secondly that hyperpolarized [1,4-¹³C₂]malate production 24 h after reperfusion correlates with renal necrosis in a 40-min unilateral ischemic rat model.

SUBMITTER: Nielsen PM 

PROVIDER: S-EPMC5240145 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature