Screening for potential genes associated with bone overgrowth after mid-shaft femur fracture in a rat model.

ABSTRACT:

Background

We investigated the underlying molecular mechanisms of bone overgrowth after femoral fracture by using high-throughput bioinformatics approaches.

Methods

The gene expression profile of GSE3298 (accession number) was obtained from the Gene Expression Omnibus database. Sixteen femoral growth plate samples, including nine samples without fracture and seven fracture samples for seven time points, were used for analysis. The Limma package was applied to identify differentially expressed genes (DEGs) between fractured and intact samples. The DAVID online tool was used for Gene ontology functional and pathway enrichment analysis. A protein-protein interaction (PPI) network established by String software was used to identify interactions between significant DEGs, and netw

SUBMITTER: Liu C 

PROVIDER: S-EPMC5240322 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature