Novel multispecific heterodimeric antibody format allowing modular assembly of variable domain fragments.

ABSTRACT: Multispecific antibody formats provide a promising platform for the development of novel therapeutic concepts that could facilitate the generation of safer, more effective pharmaceuticals. However, the production and use of such antibody-based multispecifics is often made complicated by: 1) the instability of the antibody fragments of which they consist, 2) undesired inter-subunit associations, and 3) the need to include recombinant heterodimerization domains that confer distribution-impairing bulk or enhance immunogenicity. In this paper, we describe a broadly-applicable method for the stabilization of human or humanized antibody Fv fragments that entails replacing framework region IV of a V_κ1/V_H3-consensus Fv framework with the corresponding germ-line sequence of a

SUBMITTER: Egan TJ 

PROVIDER: S-EPMC5240654 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature