Ohr plays a central role in bacterial responses against fatty acid hydroperoxides and peroxynitrite.

ABSTRACT: Organic hydroperoxide resistance (Ohr) enzymes are unique Cys-based, lipoyl-dependent peroxidases. Here, we investigated the involvement of Ohr in bacterial responses toward distinct hydroperoxides. In silico results indicated that fatty acid (but not cholesterol) hydroperoxides docked well into the active site of Ohr from Xylella fastidiosa and were efficiently reduced by the recombinant enzyme as assessed by a lipoamide-lipoamide dehydrogenase-coupled assay. Indeed, the rate constants between Ohr and several fatty acid hydroperoxides were in the 10⁷-10⁸ M^-1⋅s^-1 range as determined by a competition assay developed here. Reduction of peroxynitrite by Ohr was also determined to be in the order of 10⁷ M^-1⋅s^-1 at pH

SUBMITTER: Alegria TG 

PROVIDER: S-EPMC5240686 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature