Mosaic expression of claudins in thick ascending limbs of Henle results in spatial separation of paracellular Na+ and Mg2+ transport.

ABSTRACT: The thick ascending limb (TAL) of Henle's loop drives paracellular Na⁺, Ca²⁺, and Mg²⁺ reabsorption via the tight junction (TJ). The TJ is composed of claudins that consist of four transmembrane segments, two extracellular segments (ECS1 and -2), and one intracellular loop. Claudins interact within the same (cis) and opposing (trans) plasma membranes. The claudins Cldn10b, -16, and -19 facilitate cation reabsorption in the TAL, and their absence leads to a severe disturbance of renal ion homeostasis. We combined electrophysiological measurements on microperfused mouse TAL segments with subsequent analysis of claudin expression by immunostaining and confocal microscopy. Claudin interaction properties were examined using heterologous expression in the TJ-free

SUBMITTER: Milatz S 

PROVIDER: S-EPMC5240732 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature