Genetic subtyping of Listeria monocytogenes via multiple-locus sequence typing using iap, sigB and actA.

ABSTRACT: Pulse field gel electrophoresis (PFGE) is widely used for listeriosis surveillance. Although this technique is effective for epidemiology, the data among laboratories are inconsistent. We previously reported a method for Listeria monocytogenes subtyping combined with sequence analysis of partial iap and whole genome restriction fragment length polymorphism (RFLP) using XbaI, ClaI (BanIII) and PstI. However, distinguishing subtypes was challenging, because the output comprised complicated fragment patterns. In this study, we aimed to establish a simple genotyping method that does not depend on visual observation, rather it focuses on multi-locus sequence typing (MLST) using three genes, iap, sigB and actA. Sixty-eight strains of L. monocytogenes including EGD-e as a reference strain were in

SUBMITTER: Yoshikawa Y 

PROVIDER: S-EPMC5240762 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature