Clusterin and Pycr1 alterations associate with strain and model differences in susceptibility to experimental pancreatitis.

ABSTRACT: Acute pancreatitis has several underlying etiologies, and results in consequences ranging from mild to complex multi-organ failure. The wide range of pathology suggests a genetic predisposition for progression. We compared the susceptibility to acute pancreatitis in BALB/c and FVB/N mice, coupled with proteomic analysis, in order to identify potential protein associations with pancreatitis progression.

Methods

Pancreatitis was induced in BALB/c and FVB/N mice by administration of cerulein or feeding a choline-deficient, ethionine-supplemented (CDE) diet. Histology and changes in serum amylase were examined. Proteome profiling in cerulein-treated mice was performed using 2-dimensional differential in gel electrophoresis (2D-DIGE) followed by mass spectrometry analysis and biochemical

SUBMITTER: Iyer S 

PROVIDER: S-EPMC5240812 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature