Pathways of immediate hypothermia and leukocyte infiltration in an adjuvant-free mouse model of anaphylaxis.

ABSTRACT:

Background

Conflicting results have been obtained regarding the roles of Fc receptors and effector cells in models of active systemic anaphylaxis (ASA). In part, this might reflect the choice of adjuvant used during sensitization because various adjuvants might differentially influence the production of particular antibody isotypes.

Objective

We developed an "adjuvant-free" mouse model of ASA and assessed the contributions of components of the "classical" and "alternative" pathways in this model.

Methods

Mice were sensitized intraperitoneally with ovalbumin at weekly intervals for 6 weeks and challenged intraperitoneally with ovalbumin 2 weeks later.

Results

Wild-type animals had immediate hypothermia and late-phase intraperitoneal inflammation in this model. T

SUBMITTER: Balbino B 

PROVIDER: S-EPMC5241268 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature