A Virus-Derived Stacked RNAi Construct Confers Robust Resistance to Cassava Brown Streak Disease.

ABSTRACT: Cassava brown streak disease (CBSD) threatens food and economic security for smallholder farmers throughout East and Central Africa, and poses a threat to cassava production in West Africa. CBSD is caused by two whitefly-transmitted virus species: Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) (Genus: Ipomovirus, Family Potyviridae). Although varying levels of tolerance have been achieved through conventional breeding, to date, effective resistance to CBSD within East African cassava germplasm has not been identified. RNAi technology was utilized to integrate CBSD resistance into the Ugandan farmer-preferred cassava cultivar TME 204. Transgenic plant lines were generated expressing an inverted repeat construct (p5001) derived fr

SUBMITTER: Beyene G 

PROVIDER: S-EPMC5241307 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature