Induced Packaging of Cellular MicroRNAs into HIV-1 Virions Can Inhibit Infectivity.

ABSTRACT: Analysis of the incorporation of cellular microRNAs (miRNAs) into highly purified HIV-1 virions revealed that this largely, but not entirely, mirrored the level of miRNA expression in the producer CD4⁺ T cells. Specifically, of the 58 cellular miRNAs detected at significant levels in the producer cells, only 5 were found in virions at a level 2- to 4-fold higher than that predicted on the basis of random cytoplasmic sampling. Of note, these included two miRNAs, miR-155 and miR-92a, that were reported previously to at least weakly bind HIV-1 transcripts. To test whether miRNA binding to the HIV-1 genome can induce virion incorporation, artificial miRNA target sites were introduced into the viral genome and a 10- to 40-fold increase in the packaging of the cognate miRNAs into viri

SUBMITTER: Bogerd HP 

PROVIDER: S-EPMC5241401 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature