Unsaturated lipid bodies as a hallmark of inflammation studied by Raman 2D and 3D microscopy.

ABSTRACT: Endothelial HMEC-1 cells incubated with pro-inflammatory cytokine TNF-α for 6 and 24 hours were studied as a model of inflammation using Raman imaging. Striking changes in distribution, composition and concentration of cellular lipids were observed after exposure to TNF-α compared to the control. In particular, 3D Raman imaging revealed a significant increase in the amount of lipid entities formed under inflammation. Lipid bodies were randomly distributed in the cytoplasm and two types of droplets were assembled: more saturated one, in spectral characteristics resembling phosphatidylcholine and saturated cholesteryl esters, observed also in the control, and highly unsaturated one, containing also cholesterols, being a hallmark of inflamed cells. The statistical analysis showed that the num

SUBMITTER: Czamara K 

PROVIDER: S-EPMC5241649 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature