Optogenetic Modulation of Urinary Bladder Contraction for Lower Urinary Tract Dysfunction.

ABSTRACT: As current clinical approaches for lower urinary tract (LUT) dysfunction such as pharmacological and electrical stimulation treatments lack target specificity, thus resulting in suboptimal outcomes with various side effects, a better treatment modality with spatial and temporal target-specificity is necessary. In this study, we delivered optogenetic membrane proteins, such as channelrhodopsin-2 (ChR2) and halorhodopsin (NpHR), to bladder smooth muscle cells (SMCs) of mice using either the Cre-loxp transgenic system or a viral transfection method. The results showed that depolarizing ChR2-SMCs with blue light induced bladder contraction, whereas hyperpolarizing NpHR-SMCs with yellow light suppressed PGE₂-induced overactive contraction. We also confirmed that optogenetic contracti

SUBMITTER: Park JH 

PROVIDER: S-EPMC5241665 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature