MerR and ChrR mediate blue light induced photo-oxidative stress response at the transcriptional level in Vibrio cholerae.

ABSTRACT: Blue light (BL) is a major environmental factor that affects the physiology, behavior, and infectivity of bacteria as it contributes to the generation of reactive oxygen species (ROS) while increasing photo-oxidative stress in cells. However, precise photo-oxidative response mechanism in non-phototrophic bacteria is yet to be elucidated. In this study, we investigated the effect of BL in Vibrio cholerae by using genetics and transcriptome profiling. Genome-wide analysis revealed that transcription of 6.3% of V. cholerae genes were regulated by BL. We further showed that BL enhances ROS production, which is generated through the oxidative phosphorylation. To understand signaling mechanisms, we generated several knockouts and analyzed their transcriptome under BL exposure. Studies with a dou

SUBMITTER: Tardu M 

PROVIDER: S-EPMC5241685 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature