Characterization and phylogenetic epitope mapping of CD38 ADPR cyclase in the cynomolgus macaque.

ABSTRACT:

Background

The CD38 transmembrane glycoprotein is an ADP-ribosyl cyclase that moonlights as a receptor in cells of the immune system. Both functions are independently implicated in numerous areas related to human health. This study originated from an inherent interest in studying CD38 in the cynomolgus monkey (Macaca fascicularis), a species closely related to humans that also represents a cogent animal model for the biomedical analysis of CD38.

Results

A cDNA was isolated from cynomolgus macaque peripheral blood leukocytes and is predicted to encode a type II membrane protein of 301 amino acids with 92% identity to human CD38. Both RT-PCR-mediated cDNA cloning and genomic DNA PCR surveying were possible with heterologous human CD38 primers, demonstrating the striking conser

SUBMITTER: Ferrero E 

PROVIDER: S-EPMC524171 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature