Ontology highlight
ABSTRACT:
SUBMITTER: Zheng GX
PROVIDER: S-EPMC5241818 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Nature communications 20170116
Characterizing the transcriptome of individual cells is fundamental to understanding complex biological systems. We describe a droplet-based system that enables 3' mRNA counting of tens of thousands of single cells per sample. Cell encapsulation, of up to 8 samples at a time, takes place in ∼6 min, with ∼50% cell capture efficiency. To demonstrate the system's technical performance, we collected transcriptome data from ∼250k single cells across 29 samples. We validated the sensitivity of the sys ...[more]