A new enzyme-linked immunosorbent assay (ELISA) for human free and bound kallikrein 9.

ABSTRACT:

Background

Kallikrein 9 (KLK9) is a member of the human kallikrein-related peptidases family, whose physiological role and implications in disease processes remain unclear. The active form of the enzyme is predicted to have chymotryptic activity. In the present study, we produced for the first time the active recombinant protein and monoclonal antibodies, and developed novel immunoassays for the quantification of free and bound KLK9 in biological samples.

Methods

The coding sequence of mature KLK9 isoform (mat-KLK9) was expressed in an Expi293F mammalian system and the synthesized polypeptide was purified through a two-step protocol. The purified protein was used as an immunogen for production of monoclonal antibodies in mice. Hybridomas were further expanded and antibodies

SUBMITTER: Filippou P 

PROVIDER: S-EPMC5241945 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature