Chromatin immunoprecipitation (ChIP) scanning identifies primary glucocorticoid receptor target genes.

ABSTRACT: The global physiological effects of glucocorticoids are well established, and the framework of transcriptional regulation by the glucocorticoid receptor (GR) has been described. However, the genes directly under GR control that trigger these physiological effects are largely unknown. To address this issue in a single cell type, we identified glucocorticoid-responsive genes in A549 human lung adenocarcinoma cells by microarray analysis and quantitative real-time PCR. Reduction of GR expression by RNA interference diminished the effects of dexamethasone on all tested target genes, thus confirming the essential role of GR in glucocorticoid-regulated gene expression. To identify primary GR target genes, in which GR is a component of the transcriptional regulatory complex, we developed a strate

SUBMITTER: Wang JC 

PROVIDER: S-EPMC524211 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature