Robust, universal biomarker assay to detect senescent cells in biological specimens.

ABSTRACT: Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.

SUBMITTER: Evangelou K 

PROVIDER: S-EPMC5242262 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature