Senescence-associated DNA methylation is stochastically acquired in subpopulations of mesenchymal stem cells.

ABSTRACT: Replicative senescence has a major impact on function and integrity of cell preparations. This process is reflected by continuous DNA methylation (DNAm) changes at specific CpG dinucleotides in the course of in vitro culture, and such modifications can be used to estimate the state of cellular senescence for quality control of cell preparations. Still, it is unclear how senescence-associated DNAm changes are regulated and whether they occur simultaneously across a cell population. In this study, we analyzed global DNAm profiles of human mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs) to demonstrate that senescence-associated DNAm changes are overall similar in these different cell types. Subsequently, an Epigenetic-Senescence-Signature, based on six CpGs,

SUBMITTER: Franzen J 

PROVIDER: S-EPMC5242294 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature