Hair follicle stem cell cultures reveal self-organizing plasticity of stem cells and their progeny.

ABSTRACT: Understanding how complex tissues are formed, maintained, and regenerated through local growth, differentiation, and remodeling requires knowledge on how single-cell behaviors are coordinated on the population level. The self-renewing hair follicle, maintained by a distinct stem cell population, represents an excellent paradigm to address this question. A major obstacle in mechanistic understanding of hair follicle stem cell (HFSC) regulation has been the lack of a culture system that recapitulates HFSC behavior while allowing their precise monitoring and manipulation. Here, we establish an in vitro culture system based on a 3D extracellular matrix environment and defined soluble factors, which for the first time allows expansion and long-term maintenance of murine multipotent HFSCs in the

SUBMITTER: Chacon-Martinez CA 

PROVIDER: S-EPMC5242381 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature