A dynamic, web-accessible resource to process raw microarray scan data into consolidated gene expression values: importance of replication.

ABSTRACT: We propose a freely accessible web-based pipeline, which processes raw microarray scan data to obtain experimentally consolidated gene expression values. The tool MADSCAN, which stands for MicroArray Data Suites of Computed ANalysis, makes a practical choice among the numerous methods available for filtering, normalizing and scaling of raw microarray expression data in a dynamic and automatic way. Different statistical methods have been adapted to extract reliable information from replicate gene spots as well as from replicate microarrays for each biological situation under study. A carefully constructed experimental design thus allows to detect outlying expression values and to identify statistically significant expression values, together with a list of quality controls with proposed thr

SUBMITTER: Le Meur N 

PROVIDER: S-EPMC524282 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature