The vaa locus of Mycoplasma hominis contains a divergent genetic islet encoding a putative membrane protein.

ABSTRACT:

Background

The Mycoplasma hominis vaa gene encodes a highly variable, surface antigen involved in the adhesion to host cells. We have analysed the structure of the vaa locus to elucidate the genetic basis for variation of vaa.

Results

Mapping of vaa on existing physical maps of five M. hominis isolates by pulsed field gel electrophoresis revealed that vaa is located in a genomic region containing the majority of other characterized membrane protein genes of M. hominis. Sequencing of an 11 kb region containing the vaa locus of M. hominis isolate 132 showed the presence of conserved housekeeping genes at the borders of the region, uvrA upstream and the hitABL operon downstream to vaa. Analysis of 20 M. hominis isolates revealed that the vaa upstream region was conserved wherea

SUBMITTER: Boesen T 

PROVIDER: S-EPMC524362 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature