Isolation of suppressor genes that restore retrovirus susceptibility to a virus-resistant cell line.

ABSTRACT:

Background

Genetic selections in mammalian cell lines have recently been developed for the isolation of mutant cells that are refractory to infection by retroviruses. These selections have been used to recover lines that block early postentry stages of infection, either before reverse transcription or before nuclear entry. The mechanisms of action of these blocks remain unknown.

Results

We have devised a method for the selection of genes from cDNA libraries that suppress the block to virus infection, and so restore virus susceptibility. The protocol involves the transformation of pools of resistant cells by cDNA expression libraries, followed by the selection for rare virus-sensitive cells, using multiple rounds of selection after infection by marked viral vector genomes. Th

SUBMITTER: Gao G 

PROVIDER: S-EPMC524375 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature