Generation of α-1,3-galactosyltransferase knocked-out transgenic cloned pigs with knocked-in five human genes.

ABSTRACT: Recent progress in genetic manipulation of pigs designated for xenotransplantation ha6s shown considerable promise on xenograft survival in primates. However, genetic modification of multiple genes in donor pigs by knock-out and knock-in technologies, aiming to enhance immunological tolerance against transplanted organs in the recipients, has not been evaluated for health issues of donor pigs. We produced transgenic Massachusetts General Hospital piglets by knocking-out the α-1,3-galactosyltransferase (GT) gene and by simultaneously knocking-in an expression cassette containing five different human genes including, DAF, CD39, TFPI, C1 inhibitor (C1-INH), and TNFAIP3 (A20) [GT^{-(DAF/CD39/TFPI/C1-INH/TNFAIP3)/+}] that are connected by 2A peptide cleavage sequences to release individ

SUBMITTER: Kwon DJ 

PROVIDER: S-EPMC5243873 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature