High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic.

ABSTRACT: Here we describe the establishment of microscope-based functional screening assays in intact cells that allow us to systematically identify new proteins involved in secretory membrane traffic, and proteins that can influence the integrity of the Golgi complex. We were able to identify 20 new proteins that affected either secretory transport, Golgi morphology, or both, when overexpressed in cells. Control experiments with human orthologs to yeast proteins with a role in membrane traffic, or already well characterized mammalian regulators of the secretory pathway, confirmed the specificity and significance of our results. Proteins localized to the Golgi complex or endoplasmic reticulum (ER) showed preferential interference in our assays. Bioinformatic analysis of the new proteins interfering

SUBMITTER: Starkuviene V 

PROVIDER: S-EPMC524419 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature