Demonstration of the Protein Involvement in Cell Electropermeabilization using Confocal Raman Microspectroscopy.

ABSTRACT: Confocal Raman microspectroscopy was used to study the interaction between pulsed electric fields and live cells from a molecular point of view in a non-invasive and label-free manner. Raman signatures of live human adipose-derived mesenchymal stem cells exposed or not to pulsed electric fields (8 pulses, 1 000 V/cm, 100 μs, 1 Hz) were acquired at two cellular locations (nucleus and cytoplasm) and two spectral bands (600-1 800 cm^-1 and 2 800-3 100 cm^-1). Vibrational modes of proteins (phenylalanine and amide I) and lipids were found to be modified by the electropermeabilization process with a statistically significant difference. The relative magnitude of four phenylalanine peaks decreased in the spectra of the pulsed group. On the contrary, the relative magnitude of

SUBMITTER: Azan A 

PROVIDER: S-EPMC5244372 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature