Modification of distinct ion channels differentially modulates Ca²⁺ dynamics in primary cultured rat ventricular cardiomyocytes.

ABSTRACT: Primary cultured cardiomyocytes show spontaneous Ca²⁺ oscillations (SCOs) which not only govern contractile events, but undergo derangements that promote arrhythmogenesis through Ca²⁺ -dependent mechanism. We systematically examined influence on SCOs of an array of ion channel modifiers by recording intracellular Ca²⁺ dynamics in rat ventricular cardiomyocytes using Ca²⁺ specific fluorescence dye, Fluo-8/AM. Voltage-gated sodium channels (VGSCs) activation elongates SCO duration and reduces SCO frequency while inhibition of VGSCs decreases SCO frequency without affecting amplitude and duration. Inhibition of voltage-gated potassium channel increases SCO duration. Direct activation of L-type Ca²⁺ channels (LTCCs) induces SCO bursts whil

SUBMITTER: Li X 

PROVIDER: S-EPMC5244425 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature