Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression.

ABSTRACT:

Background

The glucose dual-affinity transport system (low- and high-affinity) is a conserved strategy used by microorganisms to cope with natural fluctuations in nutrient availability in the environment. The glucose-sensing and uptake processes are believed to be tightly associated with cellulase expression regulation in cellulolytic fungi. However, both the identities and functions of the major molecular components of this evolutionarily conserved system in filamentous fungi remain elusive. Here, we systematically identified and characterized the components of the glucose dual-affinity transport system in the model fungus Neurospora crassa.

Results

Using RNA sequencing coupled with functional transport analyses, we assigned GLT-1 (K_m = 18.42 ± 3.38

SUBMITTER: Wang B 

PROVIDER: S-EPMC5244594 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature